pet-21d(+) dna vectors (Millipore)
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pet-21d(+) dna vectors
Pet 21d(+) Dna Vectors, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-21d+vector/pet28a/bio_rxiv__2024__09__03__610925-434-22-25
Average 90 stars, based on 1 article reviews
Pet 21d(+) Dna Vectors, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-21d+vector/pet28a/bio_rxiv__2024__09__03__610925-434-22-25
Average 90 stars, based on 1 article reviews
pet-21d(+) dna vectors - by Bioz Stars,
2026-10
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Sequencing:Article Title: The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. Article Snippet: The C-terminal zinc fingers of ZBTB38 are novel selective readers of DNA methylation Article Title: Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules. Article Snippet: Constructs for bacterial expression of GST fusion proteins with N- and C-terminal extensions corresponding to the terminal sequences of the different Drosophila betatubulin paralogs were made using the pET-21d vector (Novagen). Article Title: Possible cleavage sites of glutelin partial degradation confirmed by immunological analysis in globulin-less mutants of rice (Oryza sativa L.). Article Snippet: Proteolytic cleavage or partial degradation of proteins is one of the important posttranslational modifications for various biological processes, but it is difficult to analyze.. Previously, we demonstrated that some subunits of the major rice (Oryza sativa L.) seed storage protein glutelin are partially degraded to produce newly identified polypeptides X1-X5 in mutants in which another major seed storage protein globulin is absent.. In this study, the new polypeptides X3 and X4/X5 were immunologically confirmed to be derived from GluA3 and GluA1/GluA2 subunits, respectively. Article Title: Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases. Article Snippet: New class IV transaminases with activity towards L-Leu, which is typical of branched-chain amino acid aminotransferases (BCAT), and with activity towards (R)-(+)1-phenylethylamine ((R)-PEA), which is typical of (R)-selective (R)-amine:pyruvate transaminases, were identified by bioinformatics analysis, obtained in recombinant form, and analyzed.. The values of catalytic activities in the reaction with L-Leu and (R)-PEA are comparable to those measured for characteristic transaminases with the corresponding specificity.. Earlier, (R)-selective class IV transaminases were found to be active, apart from (R)-PEA, only with some other (R)-primary amines and D-amino acids. Article Title: Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens . Article Snippet: The optimized Article Title: Functional characterization of PLP fold type IV transaminase with a mixed type of activity from Haliangium ochraceum. Article Snippet: AC C EP TE D M AN U 1 Pyridoxal-5’-phosphate (PLP)-dependent transaminases are industrially important enzymes catalyzing the stereoselective amination of ketones and keto acids.. Transaminases of PLP fold type IV are characterized by (R)- or (S)-stereoselective transfer of amino groups, depending on the substrate profile of the enzyme.. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases and (R)amine:pyruvate transaminases. Article Title: Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration Article Snippet: To construct the plasmid encoding the His-tagged ADD1 ΔHead domain (aa 350–737), cDNAs from pCMV-3Tag-3A-ADD1 ΔHead was subcloned into Article Title: Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice Article Snippet: Briefly, the cDNA for the neck/CRD, including a short region of the collagen stalk (8 Gly-X-Y) and representing residues 179–355 was cloned from human lung library DNA and inserted into a pET-21d vector (Novagen, Nottingham). Synthesized:Article Title: The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. Article Snippet: The C-terminal zinc fingers of ZBTB38 are novel selective readers of DNA methylation Article Title: Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules. Article Snippet: Constructs for bacterial expression of GST fusion proteins with N- and C-terminal extensions corresponding to the terminal sequences of the different Drosophila betatubulin paralogs were made using the pET-21d vector (Novagen). Article Title: Possible cleavage sites of glutelin partial degradation confirmed by immunological analysis in globulin-less mutants of rice (Oryza sativa L.). Article Snippet: Proteolytic cleavage or partial degradation of proteins is one of the important posttranslational modifications for various biological processes, but it is difficult to analyze.. Previously, we demonstrated that some subunits of the major rice (Oryza sativa L.) seed storage protein glutelin are partially degraded to produce newly identified polypeptides X1-X5 in mutants in which another major seed storage protein globulin is absent.. In this study, the new polypeptides X3 and X4/X5 were immunologically confirmed to be derived from GluA3 and GluA1/GluA2 subunits, respectively. Article Title: Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases. Article Snippet: New class IV transaminases with activity towards L-Leu, which is typical of branched-chain amino acid aminotransferases (BCAT), and with activity towards (R)-(+)1-phenylethylamine ((R)-PEA), which is typical of (R)-selective (R)-amine:pyruvate transaminases, were identified by bioinformatics analysis, obtained in recombinant form, and analyzed.. The values of catalytic activities in the reaction with L-Leu and (R)-PEA are comparable to those measured for characteristic transaminases with the corresponding specificity.. Earlier, (R)-selective class IV transaminases were found to be active, apart from (R)-PEA, only with some other (R)-primary amines and D-amino acids. Article Title: Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens . Article Snippet: The optimized Article Title: Functional characterization of PLP fold type IV transaminase with a mixed type of activity from Haliangium ochraceum. Article Snippet: AC C EP TE D M AN U 1 Pyridoxal-5’-phosphate (PLP)-dependent transaminases are industrially important enzymes catalyzing the stereoselective amination of ketones and keto acids.. Transaminases of PLP fold type IV are characterized by (R)- or (S)-stereoselective transfer of amino groups, depending on the substrate profile of the enzyme.. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases and (R)amine:pyruvate transaminases. Article Title: Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration Article Snippet: To construct the plasmid encoding the His-tagged ADD1 ΔHead domain (aa 350–737), cDNAs from pCMV-3Tag-3A-ADD1 ΔHead was subcloned into Article Title: Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice Article Snippet: Briefly, the cDNA for the neck/CRD, including a short region of the collagen stalk (8 Gly-X-Y) and representing residues 179–355 was cloned from human lung library DNA and inserted into a pET-21d vector (Novagen, Nottingham). Clone Assay:Article Title: The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. Article Snippet: The C-terminal zinc fingers of ZBTB38 are novel selective readers of DNA methylation Article Title: Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules. Article Snippet: Constructs for bacterial expression of GST fusion proteins with N- and C-terminal extensions corresponding to the terminal sequences of the different Drosophila betatubulin paralogs were made using the pET-21d vector (Novagen). Article Title: Possible cleavage sites of glutelin partial degradation confirmed by immunological analysis in globulin-less mutants of rice (Oryza sativa L.). Article Snippet: Proteolytic cleavage or partial degradation of proteins is one of the important posttranslational modifications for various biological processes, but it is difficult to analyze.. Previously, we demonstrated that some subunits of the major rice (Oryza sativa L.) seed storage protein glutelin are partially degraded to produce newly identified polypeptides X1-X5 in mutants in which another major seed storage protein globulin is absent.. In this study, the new polypeptides X3 and X4/X5 were immunologically confirmed to be derived from GluA3 and GluA1/GluA2 subunits, respectively. Article Title: Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases. Article Snippet: New class IV transaminases with activity towards L-Leu, which is typical of branched-chain amino acid aminotransferases (BCAT), and with activity towards (R)-(+)1-phenylethylamine ((R)-PEA), which is typical of (R)-selective (R)-amine:pyruvate transaminases, were identified by bioinformatics analysis, obtained in recombinant form, and analyzed.. The values of catalytic activities in the reaction with L-Leu and (R)-PEA are comparable to those measured for characteristic transaminases with the corresponding specificity.. Earlier, (R)-selective class IV transaminases were found to be active, apart from (R)-PEA, only with some other (R)-primary amines and D-amino acids. Article Title: Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens . Article Snippet: The optimized Article Title: Functional characterization of PLP fold type IV transaminase with a mixed type of activity from Haliangium ochraceum. Article Snippet: AC C EP TE D M AN U 1 Pyridoxal-5’-phosphate (PLP)-dependent transaminases are industrially important enzymes catalyzing the stereoselective amination of ketones and keto acids.. Transaminases of PLP fold type IV are characterized by (R)- or (S)-stereoselective transfer of amino groups, depending on the substrate profile of the enzyme.. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases and (R)amine:pyruvate transaminases. Article Title: Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration Article Snippet: To construct the plasmid encoding the His-tagged ADD1 ΔHead domain (aa 350–737), cDNAs from pCMV-3Tag-3A-ADD1 ΔHead was subcloned into Article Title: Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice Article Snippet: Briefly, the cDNA for the neck/CRD, including a short region of the collagen stalk (8 Gly-X-Y) and representing residues 179–355 was cloned from human lung library DNA and inserted into a pET-21d vector (Novagen, Nottingham). Plasmid Preparation:Article Title: The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. Article Snippet: The C-terminal zinc fingers of ZBTB38 are novel selective readers of DNA methylation Article Title: Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules. Article Snippet: Constructs for bacterial expression of GST fusion proteins with N- and C-terminal extensions corresponding to the terminal sequences of the different Drosophila betatubulin paralogs were made using the pET-21d vector (Novagen). Article Title: Possible cleavage sites of glutelin partial degradation confirmed by immunological analysis in globulin-less mutants of rice (Oryza sativa L.). Article Snippet: Proteolytic cleavage or partial degradation of proteins is one of the important posttranslational modifications for various biological processes, but it is difficult to analyze.. Previously, we demonstrated that some subunits of the major rice (Oryza sativa L.) seed storage protein glutelin are partially degraded to produce newly identified polypeptides X1-X5 in mutants in which another major seed storage protein globulin is absent.. In this study, the new polypeptides X3 and X4/X5 were immunologically confirmed to be derived from GluA3 and GluA1/GluA2 subunits, respectively. Article Title: Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases. Article Snippet: New class IV transaminases with activity towards L-Leu, which is typical of branched-chain amino acid aminotransferases (BCAT), and with activity towards (R)-(+)1-phenylethylamine ((R)-PEA), which is typical of (R)-selective (R)-amine:pyruvate transaminases, were identified by bioinformatics analysis, obtained in recombinant form, and analyzed.. The values of catalytic activities in the reaction with L-Leu and (R)-PEA are comparable to those measured for characteristic transaminases with the corresponding specificity.. Earlier, (R)-selective class IV transaminases were found to be active, apart from (R)-PEA, only with some other (R)-primary amines and D-amino acids. Article Title: Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens . Article Snippet: The optimized Article Title: Functional characterization of PLP fold type IV transaminase with a mixed type of activity from Haliangium ochraceum. Article Snippet: AC C EP TE D M AN U 1 Pyridoxal-5’-phosphate (PLP)-dependent transaminases are industrially important enzymes catalyzing the stereoselective amination of ketones and keto acids.. Transaminases of PLP fold type IV are characterized by (R)- or (S)-stereoselective transfer of amino groups, depending on the substrate profile of the enzyme.. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases and (R)amine:pyruvate transaminases. Article Title: Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration Article Snippet: To construct the plasmid encoding the His-tagged ADD1 ΔHead domain (aa 350–737), cDNAs from pCMV-3Tag-3A-ADD1 ΔHead was subcloned into Article Title: Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice Article Snippet: Briefly, the cDNA for the neck/CRD, including a short region of the collagen stalk (8 Gly-X-Y) and representing residues 179–355 was cloned from human lung library DNA and inserted into a pET-21d vector (Novagen, Nottingham). Modification:Article Title: The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. Article Snippet: The C-terminal zinc fingers of ZBTB38 are novel selective readers of DNA methylation Article Title: Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules. Article Snippet: Constructs for bacterial expression of GST fusion proteins with N- and C-terminal extensions corresponding to the terminal sequences of the different Drosophila betatubulin paralogs were made using the pET-21d vector (Novagen). Article Title: Possible cleavage sites of glutelin partial degradation confirmed by immunological analysis in globulin-less mutants of rice (Oryza sativa L.). Article Snippet: Proteolytic cleavage or partial degradation of proteins is one of the important posttranslational modifications for various biological processes, but it is difficult to analyze.. Previously, we demonstrated that some subunits of the major rice (Oryza sativa L.) seed storage protein glutelin are partially degraded to produce newly identified polypeptides X1-X5 in mutants in which another major seed storage protein globulin is absent.. In this study, the new polypeptides X3 and X4/X5 were immunologically confirmed to be derived from GluA3 and GluA1/GluA2 subunits, respectively. Article Title: Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases. Article Snippet: New class IV transaminases with activity towards L-Leu, which is typical of branched-chain amino acid aminotransferases (BCAT), and with activity towards (R)-(+)1-phenylethylamine ((R)-PEA), which is typical of (R)-selective (R)-amine:pyruvate transaminases, were identified by bioinformatics analysis, obtained in recombinant form, and analyzed.. The values of catalytic activities in the reaction with L-Leu and (R)-PEA are comparable to those measured for characteristic transaminases with the corresponding specificity.. Earlier, (R)-selective class IV transaminases were found to be active, apart from (R)-PEA, only with some other (R)-primary amines and D-amino acids. Article Title: Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens . Article Snippet: The optimized Article Title: Functional characterization of PLP fold type IV transaminase with a mixed type of activity from Haliangium ochraceum. Article Snippet: AC C EP TE D M AN U 1 Pyridoxal-5’-phosphate (PLP)-dependent transaminases are industrially important enzymes catalyzing the stereoselective amination of ketones and keto acids.. Transaminases of PLP fold type IV are characterized by (R)- or (S)-stereoselective transfer of amino groups, depending on the substrate profile of the enzyme.. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases and (R)amine:pyruvate transaminases. Article Title: Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration Article Snippet: To construct the plasmid encoding the His-tagged ADD1 ΔHead domain (aa 350–737), cDNAs from pCMV-3Tag-3A-ADD1 ΔHead was subcloned into Article Title: Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice Article Snippet: Briefly, the cDNA for the neck/CRD, including a short region of the collagen stalk (8 Gly-X-Y) and representing residues 179–355 was cloned from human lung library DNA and inserted into a pET-21d vector (Novagen, Nottingham). Construct:Article Title: The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. Article Snippet: The C-terminal zinc fingers of ZBTB38 are novel selective readers of DNA methylation Article Title: Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules. Article Snippet: Constructs for bacterial expression of GST fusion proteins with N- and C-terminal extensions corresponding to the terminal sequences of the different Drosophila betatubulin paralogs were made using the pET-21d vector (Novagen). Article Title: Possible cleavage sites of glutelin partial degradation confirmed by immunological analysis in globulin-less mutants of rice (Oryza sativa L.). Article Snippet: Proteolytic cleavage or partial degradation of proteins is one of the important posttranslational modifications for various biological processes, but it is difficult to analyze.. Previously, we demonstrated that some subunits of the major rice (Oryza sativa L.) seed storage protein glutelin are partially degraded to produce newly identified polypeptides X1-X5 in mutants in which another major seed storage protein globulin is absent.. In this study, the new polypeptides X3 and X4/X5 were immunologically confirmed to be derived from GluA3 and GluA1/GluA2 subunits, respectively. Article Title: Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases. Article Snippet: New class IV transaminases with activity towards L-Leu, which is typical of branched-chain amino acid aminotransferases (BCAT), and with activity towards (R)-(+)1-phenylethylamine ((R)-PEA), which is typical of (R)-selective (R)-amine:pyruvate transaminases, were identified by bioinformatics analysis, obtained in recombinant form, and analyzed.. The values of catalytic activities in the reaction with L-Leu and (R)-PEA are comparable to those measured for characteristic transaminases with the corresponding specificity.. Earlier, (R)-selective class IV transaminases were found to be active, apart from (R)-PEA, only with some other (R)-primary amines and D-amino acids. Article Title: Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens . Article Snippet: The optimized Article Title: Functional characterization of PLP fold type IV transaminase with a mixed type of activity from Haliangium ochraceum. Article Snippet: AC C EP TE D M AN U 1 Pyridoxal-5’-phosphate (PLP)-dependent transaminases are industrially important enzymes catalyzing the stereoselective amination of ketones and keto acids.. Transaminases of PLP fold type IV are characterized by (R)- or (S)-stereoselective transfer of amino groups, depending on the substrate profile of the enzyme.. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases and (R)amine:pyruvate transaminases. Article Title: Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration Article Snippet: To construct the plasmid encoding the His-tagged ADD1 ΔHead domain (aa 350–737), cDNAs from pCMV-3Tag-3A-ADD1 ΔHead was subcloned into Article Title: Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice Article Snippet: Briefly, the cDNA for the neck/CRD, including a short region of the collagen stalk (8 Gly-X-Y) and representing residues 179–355 was cloned from human lung library DNA and inserted into a pET-21d vector (Novagen, Nottingham). Polymerase Chain Reaction:Article Title: The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. Article Snippet: The C-terminal zinc fingers of ZBTB38 are novel selective readers of DNA methylation Article Title: Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules. Article Snippet: Constructs for bacterial expression of GST fusion proteins with N- and C-terminal extensions corresponding to the terminal sequences of the different Drosophila betatubulin paralogs were made using the pET-21d vector (Novagen). Article Title: Possible cleavage sites of glutelin partial degradation confirmed by immunological analysis in globulin-less mutants of rice (Oryza sativa L.). Article Snippet: Proteolytic cleavage or partial degradation of proteins is one of the important posttranslational modifications for various biological processes, but it is difficult to analyze.. Previously, we demonstrated that some subunits of the major rice (Oryza sativa L.) seed storage protein glutelin are partially degraded to produce newly identified polypeptides X1-X5 in mutants in which another major seed storage protein globulin is absent.. In this study, the new polypeptides X3 and X4/X5 were immunologically confirmed to be derived from GluA3 and GluA1/GluA2 subunits, respectively. Article Title: Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases. Article Snippet: New class IV transaminases with activity towards L-Leu, which is typical of branched-chain amino acid aminotransferases (BCAT), and with activity towards (R)-(+)1-phenylethylamine ((R)-PEA), which is typical of (R)-selective (R)-amine:pyruvate transaminases, were identified by bioinformatics analysis, obtained in recombinant form, and analyzed.. The values of catalytic activities in the reaction with L-Leu and (R)-PEA are comparable to those measured for characteristic transaminases with the corresponding specificity.. Earlier, (R)-selective class IV transaminases were found to be active, apart from (R)-PEA, only with some other (R)-primary amines and D-amino acids. Article Title: Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens . Article Snippet: The optimized Article Title: Functional characterization of PLP fold type IV transaminase with a mixed type of activity from Haliangium ochraceum. Article Snippet: AC C EP TE D M AN U 1 Pyridoxal-5’-phosphate (PLP)-dependent transaminases are industrially important enzymes catalyzing the stereoselective amination of ketones and keto acids.. Transaminases of PLP fold type IV are characterized by (R)- or (S)-stereoselective transfer of amino groups, depending on the substrate profile of the enzyme.. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases and (R)amine:pyruvate transaminases. Article Title: Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration Article Snippet: To construct the plasmid encoding the His-tagged ADD1 ΔHead domain (aa 350–737), cDNAs from pCMV-3Tag-3A-ADD1 ΔHead was subcloned into Article Title: Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice Article Snippet: Briefly, the cDNA for the neck/CRD, including a short region of the collagen stalk (8 Gly-X-Y) and representing residues 179–355 was cloned from human lung library DNA and inserted into a pET-21d vector (Novagen, Nottingham). Amplification:Article Title: The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. Article Snippet: The C-terminal zinc fingers of ZBTB38 are novel selective readers of DNA methylation Article Title: Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules. Article Snippet: Constructs for bacterial expression of GST fusion proteins with N- and C-terminal extensions corresponding to the terminal sequences of the different Drosophila betatubulin paralogs were made using the pET-21d vector (Novagen). Article Title: Possible cleavage sites of glutelin partial degradation confirmed by immunological analysis in globulin-less mutants of rice (Oryza sativa L.). Article Snippet: Proteolytic cleavage or partial degradation of proteins is one of the important posttranslational modifications for various biological processes, but it is difficult to analyze.. Previously, we demonstrated that some subunits of the major rice (Oryza sativa L.) seed storage protein glutelin are partially degraded to produce newly identified polypeptides X1-X5 in mutants in which another major seed storage protein globulin is absent.. In this study, the new polypeptides X3 and X4/X5 were immunologically confirmed to be derived from GluA3 and GluA1/GluA2 subunits, respectively. Article Title: Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases. Article Snippet: New class IV transaminases with activity towards L-Leu, which is typical of branched-chain amino acid aminotransferases (BCAT), and with activity towards (R)-(+)1-phenylethylamine ((R)-PEA), which is typical of (R)-selective (R)-amine:pyruvate transaminases, were identified by bioinformatics analysis, obtained in recombinant form, and analyzed.. The values of catalytic activities in the reaction with L-Leu and (R)-PEA are comparable to those measured for characteristic transaminases with the corresponding specificity.. Earlier, (R)-selective class IV transaminases were found to be active, apart from (R)-PEA, only with some other (R)-primary amines and D-amino acids. Article Title: Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens . Article Snippet: The optimized Article Title: Functional characterization of PLP fold type IV transaminase with a mixed type of activity from Haliangium ochraceum. Article Snippet: AC C EP TE D M AN U 1 Pyridoxal-5’-phosphate (PLP)-dependent transaminases are industrially important enzymes catalyzing the stereoselective amination of ketones and keto acids.. Transaminases of PLP fold type IV are characterized by (R)- or (S)-stereoselective transfer of amino groups, depending on the substrate profile of the enzyme.. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases and (R)amine:pyruvate transaminases. Article Title: Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration Article Snippet: To construct the plasmid encoding the His-tagged ADD1 ΔHead domain (aa 350–737), cDNAs from pCMV-3Tag-3A-ADD1 ΔHead was subcloned into Article Title: Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice Article Snippet: Briefly, the cDNA for the neck/CRD, including a short region of the collagen stalk (8 Gly-X-Y) and representing residues 179–355 was cloned from human lung library DNA and inserted into a pET-21d vector (Novagen, Nottingham). cDNA Library Assay:Article Title: The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. Article Snippet: The C-terminal zinc fingers of ZBTB38 are novel selective readers of DNA methylation Article Title: Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules. Article Snippet: Constructs for bacterial expression of GST fusion proteins with N- and C-terminal extensions corresponding to the terminal sequences of the different Drosophila betatubulin paralogs were made using the pET-21d vector (Novagen). Article Title: Possible cleavage sites of glutelin partial degradation confirmed by immunological analysis in globulin-less mutants of rice (Oryza sativa L.). Article Snippet: Proteolytic cleavage or partial degradation of proteins is one of the important posttranslational modifications for various biological processes, but it is difficult to analyze.. Previously, we demonstrated that some subunits of the major rice (Oryza sativa L.) seed storage protein glutelin are partially degraded to produce newly identified polypeptides X1-X5 in mutants in which another major seed storage protein globulin is absent.. In this study, the new polypeptides X3 and X4/X5 were immunologically confirmed to be derived from GluA3 and GluA1/GluA2 subunits, respectively. Article Title: Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases. Article Snippet: New class IV transaminases with activity towards L-Leu, which is typical of branched-chain amino acid aminotransferases (BCAT), and with activity towards (R)-(+)1-phenylethylamine ((R)-PEA), which is typical of (R)-selective (R)-amine:pyruvate transaminases, were identified by bioinformatics analysis, obtained in recombinant form, and analyzed.. The values of catalytic activities in the reaction with L-Leu and (R)-PEA are comparable to those measured for characteristic transaminases with the corresponding specificity.. Earlier, (R)-selective class IV transaminases were found to be active, apart from (R)-PEA, only with some other (R)-primary amines and D-amino acids. Article Title: Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens . Article Snippet: The optimized Article Title: Functional characterization of PLP fold type IV transaminase with a mixed type of activity from Haliangium ochraceum. Article Snippet: AC C EP TE D M AN U 1 Pyridoxal-5’-phosphate (PLP)-dependent transaminases are industrially important enzymes catalyzing the stereoselective amination of ketones and keto acids.. Transaminases of PLP fold type IV are characterized by (R)- or (S)-stereoselective transfer of amino groups, depending on the substrate profile of the enzyme.. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases and (R)amine:pyruvate transaminases. Article Title: Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration Article Snippet: To construct the plasmid encoding the His-tagged ADD1 ΔHead domain (aa 350–737), cDNAs from pCMV-3Tag-3A-ADD1 ΔHead was subcloned into Article Title: Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice Article Snippet: Briefly, the cDNA for the neck/CRD, including a short region of the collagen stalk (8 Gly-X-Y) and representing residues 179–355 was cloned from human lung library DNA and inserted into a pET-21d vector (Novagen, Nottingham). Expressing:Article Title: The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. Article Snippet: The C-terminal zinc fingers of ZBTB38 are novel selective readers of DNA methylation Article Title: Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules. Article Snippet: Constructs for bacterial expression of GST fusion proteins with N- and C-terminal extensions corresponding to the terminal sequences of the different Drosophila betatubulin paralogs were made using the pET-21d vector (Novagen). Article Title: Possible cleavage sites of glutelin partial degradation confirmed by immunological analysis in globulin-less mutants of rice (Oryza sativa L.). Article Snippet: Proteolytic cleavage or partial degradation of proteins is one of the important posttranslational modifications for various biological processes, but it is difficult to analyze.. Previously, we demonstrated that some subunits of the major rice (Oryza sativa L.) seed storage protein glutelin are partially degraded to produce newly identified polypeptides X1-X5 in mutants in which another major seed storage protein globulin is absent.. In this study, the new polypeptides X3 and X4/X5 were immunologically confirmed to be derived from GluA3 and GluA1/GluA2 subunits, respectively. Article Title: Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases. Article Snippet: New class IV transaminases with activity towards L-Leu, which is typical of branched-chain amino acid aminotransferases (BCAT), and with activity towards (R)-(+)1-phenylethylamine ((R)-PEA), which is typical of (R)-selective (R)-amine:pyruvate transaminases, were identified by bioinformatics analysis, obtained in recombinant form, and analyzed.. The values of catalytic activities in the reaction with L-Leu and (R)-PEA are comparable to those measured for characteristic transaminases with the corresponding specificity.. Earlier, (R)-selective class IV transaminases were found to be active, apart from (R)-PEA, only with some other (R)-primary amines and D-amino acids. Article Title: Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens . Article Snippet: The optimized Article Title: Functional characterization of PLP fold type IV transaminase with a mixed type of activity from Haliangium ochraceum. Article Snippet: AC C EP TE D M AN U 1 Pyridoxal-5’-phosphate (PLP)-dependent transaminases are industrially important enzymes catalyzing the stereoselective amination of ketones and keto acids.. Transaminases of PLP fold type IV are characterized by (R)- or (S)-stereoselective transfer of amino groups, depending on the substrate profile of the enzyme.. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases and (R)amine:pyruvate transaminases. Article Title: Phosphorylation of adducin-1 by cyclin-dependent kinase 5 is important for epidermal growth factor-induced cell migration Article Snippet: To construct the plasmid encoding the His-tagged ADD1 ΔHead domain (aa 350–737), cDNAs from pCMV-3Tag-3A-ADD1 ΔHead was subcloned into Article Title: Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice Article Snippet: Briefly, the cDNA for the neck/CRD, including a short region of the collagen stalk (8 Gly-X-Y) and representing residues 179–355 was cloned from human lung library DNA and inserted into a pET-21d vector (Novagen, Nottingham). |